ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (6): 746-754.doi:

• 预防兽医 • Previous Articles     Next Articles

Genetic Relationship and Sequence Variation of Haemaphysalis longicornis and H. conicinna based on ITS-2, COⅠ and COⅡ Gene Sequences

GU Xiao-bin1#, YU Zeng-ying1#, YANG Guang-you1*, SUN Jia-gang2,
WEI-Hong2, LI Kai-jun2, WANG Shu-xian1

  

  1. 1. College of Veterinary Medicine, Sichuan Agricultural University, Ya′an 625014, China;2. Cangxi Animal Husbandry Bureau, Cangxi 628400, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-06-25 Published:2010-06-25

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Abstract: This experiment was conducted to clarify the genetic relationship between Haemaphysalis longicornis and H. conicinna. The second internal transcribed spacer (ITS-2) of the rRNA gene, cytochrome c oxidase subunit Ⅰ (COⅠ) and cytochrome c oxidase subunit Ⅱ (COⅡ) of the mitochondrial gene were sequenced. Then, the nucleotide variability of the genes between the two tick species was compared and phylogenetic relationship was analyzed. The lengths of ITS-2, COⅠ and COⅡ in H. longicornis were 361, 479 and 647 bp, and those in H. conicinna were 354, 474 and 661 bp, respectively. The gene homology of ITS-2, COⅠ and COⅡ between H. longicornis and H. conicinna were 84.2%, 89.0% and 88.4%, respectively. Phylogenetic trees reconstructed using the NJ method based on genes ITS-2, COⅠ and COⅡ indicate that H. longicornis and H. conicinna are clustered in the same clade. The results suggest that the length of ITS-2, COⅠ and COⅡ of H. longicornis and H. conicinna are different, and H. longicornis and H. conicinna should be two different valid species with close relationship in genetics, which supports the morphologic taxonomic status of H. longicornis and H. conicinna.