ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (6): 671-677.doi:

• 遗传繁育 • Previous Articles     Next Articles

Expression of Growth Differentiation Factor 9 in Goat COCs during in vitro Maturation

ZHENG Ai-yan1,2, WU Man1, SHANG Li-qing1, DING Jia-tong1*   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.The Center for Reproduction and Heredity, Suzhou Medicine Hospital, Suzhou 225000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-06-25 Published:2010-06-25

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Abstract: To analyze the relationship between GDF9 gene and expansion of cumulusoocyte complex (COCs), the expression of GDF9 in goat COCs during oocytes in vitro maturation (IVM) (0, 6, 12, 18, 24 and 27 h) was detected by fluorescent quantitative RTPCR method. The result showed that GDF9 mRNA were expressed at low levels in immature oocytes and increased to the highest level at 12 h of IVM, which coincides with the time of cumulus cell expansion, then declined slowly during IVM, with a slight increase at the end of IVM. The expression pattern of GDF9 suggested that it might be important in cumulus expansion and oocyte maturation of goat.