Abstract: The objective of this study was to analyse the promoter structure and activity of the chicken lipoprotein lipase (LPL) gene. 2 kb 5′ flanking region of chicken LPL gene was amplified by PCR, cloned and sequenced. Subsequently, the luciferase reporter gene plasmids containing LPL gene promoter and its serial promoter deletions were constructed and introduced into chicken fibroblast cell line DF-1. The expression of luciferase was measured in transient expression assays. Bioinformatics analysis showed that the promoter fragment contained binding sites of transcription factors Oct1, GC box, CCAAT, GATA, AP1 and it also had a CpG island from －575 to +137 bp. Luciferase reporter assays demonstrated that the promoter region from －359 to +163 bp confered basal transcriptional activity and the region from －601 to +163 bp confered the most powerful transcriptional activity of the chicken LPL gene. These results indicated that chicken lipoprotein lipase gene could be regulated by variety of transcription factors and upstream elements. This study pays the way for further research on the regulation mechanism of chicken LPL gene.