Abstract: The study was aimed at cloning porcine Cyclin A gene and expressing it in swine umbilicus veins endothelial cell (SUVEC) to verify its functionality. The human Cyclin A gene was utilized as the informational probe to eclone the pigs Cyclin A gene. Reverse transcription polymerase chain reaction (RTPCR) was used to identify the products in silico cloned, and the bioinformatics methods was used to analyze structure characteristics of the gene, RTPCR, Western blot were employed for investigating its expression in SUVEC. Subcellular localization of Cyclin A was analyzed by confocal microscope, and the flow cytometry was used to analyze the cell cycle, and MTS was used to detect the proliferation of the cells. The results proved that the product of RTPCR was consistent with that of silico cloning. This cDNA contains the complete open reading frame (ORF) of 1 299 bp coding 432 amino acid residues，and NCBI BLAST analysis indicated that the gene is located in swine on chromosome 8. Western blot manifested that molecular size of Cyclin A protein was about 40 kDa, and subcellular localization showed that this protein was localized in nuclear. The flow cytometry analysis showed that cell lines SUVECCycAGFP of G1 phase cells increased by 15% to 20%, while those in S phase decreased about 18% compared with that of the control cells. MTS assay showed that the proliferative activity of cell lines SUVECCycAGFP was significantly higher than that of the control groups. Our results indicate that Cyclin A gene of pig was successfully cloned and its biological function was also confirmed, which will provide a foundation for further research on the impact of virus infection on the Cyclin A.