ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (3): 366-370.doi:

• 研究简报 • Previous Articles     Next Articles

Effect of Ovulation Status on Efficiency of the Transplant in Recipient Gilts Transferred with Somatic Cloned Embryos

LUO Xueming 1, 2, ZHANG Weihong 1, 2, FENG Chong 2, LONG Chuan 2, YAN Jun 2,ZHAO Huijing 2, ZHANG Zhaowang 1, ZHANG Zijun 1,3, PAN Dengke 1, 2*
   

  1. 1. Gansu Key Laboratory of Herbivorous Animal Biotechnology,College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China；2. The Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China；3. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
  • Received:2009-03-03 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24
  • Contact: PAN Dengke

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Abstract: In this study, the effect of different ovulation status on pregnancy rates in surrogate recipient gilts receiving somatic nuclear transfer embryos was evaluated, in order to determine the optimal stage of the estrus cycle to achieve the highest pregnancy rates. Porcine somatic cell cloned embryos on 2 d in vitro were transplanted into the 12 d estrous recipient gilts. Recipient gilts were divided into two categories according to follicular development and ovulation status: just prior to ovulation or ovulating (Group 1); perovulation or after ovulation（Group 2）. The results were as follows：(1) pregnancy rate on 30 d of six recipient gilts from Group 1 was 100%; and the pregnancy rate on 30 d of ten recipient gilts from Group 2 was 40%, the data significantly different between Group 1 and Group 2 (P<0.05); (2) For the fullterm pregnancy rate of recipient gilts, extremely significant difference was observed between Group 1 and Group 2 (100% vs. 20%, P<0.01). These results suggested that development stage of porcine somatic cloning embryos on 2 d in vitro with recipient gilt′s womb environment of ovulation status of just prior to ovulation or ovulating was the best in the same period. It may achieve the best efficiency of embryo transfer.