ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (3): 360-365.doi:

• 基础兽医 • Previous Articles     Next Articles

Effect of the Disequilibrium of Calcium Homeostasis on the Apoptosis of the Neurons in the Chicken Brain Induced by Manganese in Vitro

CHEN Lei 1, LI Zhipeng 1,2, LI Shu 1, XU Shiwen 1*
   

  1. 1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;2. Linghe Animal Husbandry and Veterinary Station, Anqiu 262100, China
  • Received:2009-11-06 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24
  • Contact: XU Shiwen

PDF (PC)

588

Abstract: To study the effect of the disequilibrium of calcium homeostasis on the apoptosis of the neurons in the chicken brain induced by manganese in vitro, neurons cultured in vitro were chosen as the research object. Neurons were cultured in the DMEM for 24 h with the final concentration of 0, 1.5, 2 and 2.5 mmol·L-1 MnCl2. The mitochondrial membrane potential (ΔΨm) and phosphatidylserine (PS) were assessed by flow cytometry. DNA fragmentations were detected by DNA Ladder. The calcium ion concentration within neurons (［Ca2+］i) was detected using the Fura3/AM as the probe and expression level of intracellular CaM mRNA was detected by FQPCR. The results showed that ΔΨm tended to decline the expansion of the pore, DNA fragmentations and ［Ca2+］i were increased. Moreover, the expression of CaM mRNA was decreased. This study indicated that MnCl2 could decrease the activity of CaM, ΔΨm and increase ［Ca2+］ result in apoptosis of the chicken neurons.