ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (3): 347-352.doi:

• 基础兽医 • Previous Articles     Next Articles

Effects of Apoptosis and NO Level of AVM on King Pigeon Brain Cells in Vitro Culture

LI Shu 1*, CUI Yali 1, WANG Min 1,2, XU Shiwen 1, WANG Junjie 1
   

  1. 1.College of Veterinary Medicine, Northeast Agricultural University,Harbin 150030, China;2.Heilongjiang Vocational College of Animal Husbandry andVeterinary Science, Shuangcheng 150100, China
  • Received:2009-11-04 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24
  • Contact: LI Shu

PDF (PC)

586

Abstract: The effects of apoptosis and NO level were explored with model of AVM on king pigeon brain neurocytes in vitro culture. Apoptosis was observed by TUNEL, the relative survival rate of neurocytes was detected by MTT, the percentage of apoptotic cells was detected by Flow cytometry, and the production of NO in cells was acquired by absorption spectrometry. The results showed that AVM can induce apoptosis and cytonecrosis of neurocyte, and has increased with the dose of AVM. AVM can reduced the production of NO, too, but no doseresponse relationship.