Abstract: In order to investigate the distribution of ghrelinimmunopositive cells in chickens, some samples of broiler chickens aged 2, 16, 30, 44 and 58 d were collected and studied by immunohistochemistry and microscopic image analysis. The ghrelinimmunopositive reaction was found in the glandular stomach, intestine, heart, liver, spleen, lung, kidney, pancreas, pituitary, adrenal gland, thymus, and bursa of Fabricius. The types of ghrelinimmunopositive cells (ghrelinic) were as follows: some endocrine cells and nerve plexes in the glandular stomach and intestine; Purkinje fibers in heart; Kupffer′s cells in liver; macrophages and reticular cells in spleen; pulmonary macrophages; podocytes, Intraglomerular mesangial cells, Juxtaglomerular cells in kidney; pancreatic islets cells in pancreas; some acidophilic cells and basophilic cells in pituitary; chromaffin cells in adrenal gland; thymic epithelial cells, thymic corpuscles and macrophages in thymus; some endocrine cells, reticular cells, macrophages and epithelial cells in bursa of Fabricius. With the chicken growth, the expressive contents of ghrelin were increased from aged 2 to 30 d, but slightly decreased at aged 44 and 58 d in various organs of chickens.