ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (3): 322-328.doi:

• 预防兽医 • Previous Articles     Next Articles

Cloning and Sequence Analysis of Viable but Nonculturable State Related Genes in Escherichia coli

LI Ying 1, DUAN Rui 2, WANG Weili 3, SUN Xiaoyuan 1, QIAN Aidong 1*
   

  1. 1.College of Animal Science and Technology, Jilin Agricultural University,Changchun 130118, China; 2.Liaoyuan Animal Husbandry Stationary, Liaoyuan 136200, China;3.Jilin EntryExit Inspection and Quarantine Bureau, Changchun 130062, China
  • Received:2009-11-02 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24

PDF (PC)

530

Abstract: This experiment was conducted to study the molecular mechanisms of bacteria in viable but nonculturable (VBNC) state. Three differential fragments(A1, A2 and C1) were cloned from E. coli in VBNC induced by acetic acid and 4 ℃ with mRNA differential display PCR. These fragments were all extracted from E. coli in VBNC by reverse northern hybridization. The nucleotide homology between three sequences and E. coli 23S rRNA genes was 98%, 98% and 99%, respectively; and amino acids homology were all above 97%. These results indicated that normal E. coli without any selective pressure, its RNA transcript was lower, and some 23S rRNA genes were inhibited. But when in VBNC, some related genes expression were higher than that of normal state. These genes maybe participate in transcription or protein synthesis of E. coli in VBNC.