ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (3): 301-304.doi:

• 预防兽医 • Previous Articles     Next Articles

Imunohistochemical Study on PrP Expression in Different issues of Tan Sheep in Ningxia

XU Lihua 1 ，2 ，KANG Xiaodong 3，TU Jian 1 ，ZHANG Zhuming 1，2，YANG Jianmin 1，ZHOU Xiangmei 1，YIN Xiaomin 1，YANG Lifeng 1，ZHAO Deming 1*
   

  1. 1.National Animal Transmissible Spongiform Encephalopathies Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China；2.College of Agriculture,Ningxia University, Yinchuan 750021, China；3.Technology Center of Animal Products,Ningxia Academy of Agriculture and Forestry Sciences， Yinchuan 750004, China
  • Received:2009-09-15 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24
  • Contact: ZHAO Deming

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Abstract: In the present experiment, a qualitative detection was implemented to determine distribution and location of prion protein（PrP） in different tissues of Ningxia Tan sheep, which is one of the protected species of classⅠ. The result indicated that PrP has a wide range of expression in different tissues, such as cerebrum, brain stem, cerebellum, heart, liver, spleen, kidney, lymph node and so on. Especially, prion protein has a higher expression level in brain stem, spleen, kidney and lymph node than other tissues. We also got a negative result in the samples from lung. This study confirmed the status of distribution of PrP in different tissues from Tan sheep. In addition, the study provided a technical support to diagnose and detect scrapie by optimizing immunohistochemical method.