ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (3): 274-278.doi:

• 遗传繁育 • Previous Articles     Next Articles

Cloning and Analyzing the Promoter of Mouse TLE4 Gene

ZHAO Lili1,2, MU Yulian 2*, CUI Wentao2, YANG Shulin2, TANG Zhonglin2,LI Yong2, ZHAO Weimin2, LIU Di 1, LI Kui 2
   

  1. 1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China; 2. Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2009-03-05 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24
  • Contact: MU Yulian

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Abstract: The preliminary objective of the study was to investigate the transcriptional regulatory mechanisms of mouse TLE4.5′upstream promoter sequences (spanned from -2 521 bp to +327 bp) of mouse TLE4 gene were amplified by PCR. Seven promoter fragments with different length were obtained by walking deletion and then cloned into luciferase report gene expression vectors. The vector expression activities were determined by transfection of the mouse F9 teratoma cells and the mouse embryonic stem cells with the constructed dualluciferase vectors.The experiment results indicated that negative regulation elements were localized within the promotor region from -2 521 bp to -2 137 bp, and further deletion analysis indicated that the region from -2 027 bp to -1 927 bp in TLE4 gene promotor showed higher activity than other regions. In addition, a functional HSF regulation element was identified in this region. The results inferred that the HSF played an important role in the regulation of TLE4 gene expression and its functional performance.