ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (3): 262-267.doi:

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A Typing System Based on Ligase Detection Reaction for Chicken Meat Flavor Related Candidate Genes Polymorphism

LI Huifang 1, SHU Jingting 1, ZHANG Ying 2, KUANG Zhixiang 2, ZHU Wenqi 1,HAN Wei 1, CHEN Kuanwei 1*   

  1. 1. Institute of Poultry Science，Chinese Academy of Agricultural Sciences, Yangzhou 225003, China；2. Guangdong Tiannong Food Ltd, Qingyuan 511827, China
  • Received:2009-04-07 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24
  • Contact: CHEN Kuanwei

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Abstract: To establish a parallel typing system based on ligase detection reaction (LDR) detecting the polymorphisms of AFABP, ExFABP and ADSL genes, which were all candidate genes for chicken meat flavor, and to analyze the distribution differences of different genotypes in Qingyuan partridge chickens and Recessive White chickens, 165 hen samples and 185 hen samples were collected from Recessive White chicken and Qingyuan partridge chicken, respectively. The genotypes of AFABP 51C/T, ExFABP 1011T/C and ADSL 3484C/T were identified by using PCRLDR. The results by this method were coincidence with direct sequencing. Three SNPs all exhibited medium polymorphism. The allele distribution in different breeds was different, and the difference in AFABP 51C/T locus was significant. The results indicated that the parallel typing system based on ligase detection reaction is a accurate and sensitive method to detect SNPs, and will lay the research foundation for the polymorphisms of candidate genes relating to meat flavor.