ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (10): 1296-1300.doi:

• 预防兽医 • Previous Articles     Next Articles

Establishment of the Diagnostic Method of IFAT for Cryptosporidium parvum Using the Recombinant Lactobacillus casei Expressing C. parvum P23

Geriletu1，2， SHI Quan1, WANG Yan-xia1, LAN Li3, MAN Da1, ZHANG He-ping2*   

  1. 1. College of Veterinary Medicine, Inner Mongolia Agricultural University， Hohhot 010018, China;2. Key Laboratory of Dairy Biotechnology and Engineering of Ministry of Education, Inner Mongolia Agricultural University， Hohhot 010018, China；3. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-25 Published:2010-10-25

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Abstract: The present experiment was performed with the objective of establishing the diagnostic methods of IFAT and ELISA for detection of the Cryptosporidium parvum. The prokaryotic expression vector pGEX-P23 was constructed by DNA recombinant technology and expression of the GST-P23 fusion protein by IPTG induction. After purification, the fusion protein was used as coating antigen in the ELISA. Using the same method, the recombinant vector pMGP23 has been constructed and electrotransformed into the Lactobacillus casei, named as recombinant Lactobacillus casei, which was used as a known antigen in IFAT. 508 samples of bovine serum, 187 samples of sheep serum and 197 samples of goat serum collected from the animals in Inner Mongolia were detected by ELISA and IFAT. The positive rates were 0.59%(bovine), 5.88%(sheep) and 4.57%(goat) by ELISA and 0.19%, 5.88% and 4.57% by IFAT. Accordance rate of these two diagnostic methods in bovine serum samples was 33.33% and the rate in sheep and goat was 100%. The results showed that the recombinant P23 has a good reactionogenicity. The positive results of two diagnostic methods were basically identical, which showed good identity and sensitivity. The methods could be widely applied in the diagnosis of Ｃryptosporidiosis.