Abstract: The present experiment was performed to determine whether a recombinant expression plasmid of IFN-γ and CpG ODN co-administrated with FMD antigen can enhance the immunological response. The mice were vaccinated intramuscularly with the conventional FMD antigen along with a recombinant plasmid inserted porcine IFN-γ gene and CpG ODN sequence. Specific antibody and neutralizing antibody of FMDV，lymphocyte proliferation，specific cytotoxic T lymphocyte (CTL) reaction and the secretory cytokines were detected. Adjuvant effect of the combination compared with CpG ODN alone on the antigen was able to elicit much stronger humoral immunity and cellular immunity in mice vaccinated，especially increase the neutralizing antibody level and CTL roles. Porcine IFN-γ and CpG ODN can enhance the immunological response against the FDMV antigen in mice，the recombinant plasmid of porcine IFN-γ and CpG ODN is potential to be an alternative adjuvant for the future FMD vaccine.