Abstract: The potential of using recombinant MSTN as vaccine to improve muscle mass was explored in this study. Firstly, the recombinant myostatin Cdomain was obtained through prokaryotic expression and purification. Then female Kunming White mice after immunization with recombinant Cdomain were mated with male mice. The indirect ELISA assay was used to detect the titer of antibody against MSTN in mother mice. The body weights of mice were evaluated each week. The results showed: (1) The body weight of mother mice in experiment group didn′t change significantly compared with that in control group (P>0.05). But during the late pregnancy (the 11th week after immunization), the increase of body weight of experiment group exceeded significantly that in control group (P<0.05). The body weight was (56.23±3.37)g in experiment group，and (54.11±3.18)g in control group. In the 12th week after immunization, the body weight of experiment group was (79.16±3.72)g, and that of control group was (7523±344)g. The former was significantly higher than the latter (P<0.01). After the delivery, the difference of body weight in two groups disappeared again (P>0.05); (2) The body weight of neonatal mice in experiment group was 25.8% higher than that in control group (P<0.01), but this difference decreased with the development of neonatal mice. These results proved that immunization of mother mice with recombinant MSTN Cdomain protein could significantly increase the body weight of neonatal mice.