ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (9): 1410-1413.doi:

• 研究简报 • Previous Articles     Next Articles

Cloning and Identification of MicroRNA from Bovine Alveolar Macrophage

XU Guangxian, ZHANG Yan, JIA Hao, ZHOU Ping, WANG Yujiong*
  

  1. College of Life Science, Ningxia University, Yinchuan 750021, China
  • Received:2008-11-04 Revised:1900-01-01 Online:2009-09-24 Published:2009-09-24
  • Contact: WANG Yujiong

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Abstract:

MicroRNAs (miRNAs) are a class of noncoding singlestranded RNA that play vital roles in many biological processes by regulating the expression of genes. To study the antibacterial mechanism of microRNAs in bovine alveolar macrophage, a cDNA library of microRNAs was constructed. By sequencing, 22 microRNAs have been screened in 438 clones. There are 19 microRNAs which have not been reported in miRBase, and 8 of them were sequence homology with human and rat. They are respectively named BtamiR141, BtamiR187, BtamiR191, BtamiR448, BtamiR589, BtamiR873, BtamiR463 and BtamiR562 by the rules. 11 others have not been found in any miRBase and should be further studied.