ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (7): 992-998.doi:

• 遗传繁育 • Previous Articles     Next Articles

Isolation, Sequence Analysis and Expression Profile of IDH1 in Goose Liver

ZHAO Ayong 1, 2 , CHEN Guohong 1* , LU Lizhi 3* , ZHOU Qi 2   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 2. Department of Animal Science, Zhejiang Forestry University, Linan 311300, China;3. Institute of Animal Science and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-24 Published:2009-07-24

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Abstract: To obtain an understanding of fattening mechanism of fatty liver, mRNA differential display reverse transcription PCR (DDRTPCR) was applied to study the differences of gene expression in French Landes Grey goose and Xupu White goose in different conditions of overfeeding and normal feeding. One gene was found to be significantly downregulated in fatty liver in both breeds（P<005）, which has a CDS length of 1 269 bp and 95% identity to chicken IDH1. The sequence analysis revealed that its 1 248 bpinlength open reading frame (ORF) encodes a 415aminoacids protein containning a putative conserved domain of Icd and has high homology with chicken(99%), rat(89%), human(90%), monkey(90%), cattle(88%),and mouse(88%)，respectively. The structure and function of this protein were analyzed using bioinformatics methods.The tissue expression analysis indicated that goose IDH1 mRNA was higher expressed in liver than that in other tested tissues. The present results suggested that overfeeding could decrease the mRNA expression level of goose IDH1.