ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (7): 1082-1087.doi:

• 基础兽医 • Previous Articles     Next Articles

Effects of Verapamil on the Expression of cfos and cjun in Cardiac Myocytes of the Ascites Syndrome in Broilers

LI Kai 1,2, MA Liqin 1, XU Tong 1,2, WANG Jianlin 2, WANG Huiyu 2, QIAO Jian 2*
   

  1. 1. College of Animal Science and Technology, Hebei North University, Zhangjiakou 075131, China; 2. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-24 Published:2009-07-24
  • Contact: QIAO Jian

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Abstract: Right ventricular hypertrophy and failure is an important factor in the development of ascites syndrome. In this study, the technology of immunohistochemistry and in situ hybridization were adopted to observe the changes of cfos and cjun in cardiac myocytes of broilers under low temperature and treated with verapamil. At the same time, the mechanism of cfos and cjun in right ventricular hypertrophy and failure was discussed. Compared with the controls, the broilers under low temperature showed that the average optical density of cfos/cjun protein and mRNA of right ventricular myocytes significantly increased on 22, 29, 36, 43 and 50 days of age, and the percentage of positive area about cfos/cjun mRNA expression also significantly increased. Compared with broilers under low temperature, the broilers given verapamil showed that the average optical density of cfos/cjun protein and mRNA of right ventricular myocytes significantly decreased on 22, 29, 36, 43 and 50 days of age, and the percentage of positive area about cfos/cjun mRNA expression also significantly decreased. These date demonstrated that cfos and cjun are important factors in right ventricular hypertrophy and failure. The verapamil can inhibit the expression of cfos and cjun, and prevent right ventricular hypertrophy and failure.