Abstract: The complete 3 197 open reading frames (ORFs) of the Brucella melitensis’s whole genome sequence has been analyzed. Among them, there are 191 proteins with Nterminal signal peptides based on the combination of prediction algorithms SignalP v30 and TatP, transmembrane domains prediction algorithms TMHMM v20 and Phobius. And there are also 391 nonsignal peptide secretion proteins based on the analyzed by SecretomeP. The functions of most of these proteins have not been identified clearly. For the important effect of secreted proteins have in bacteria pathology, the research of secreted proteins of brucella would be very useful to understand the pathogenic mechanism of brucella and to develope new methods for diagnosis.