ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (7): 1013-1018.doi:

• 遗传繁育 • Previous Articles     Next Articles

The Influence of SelW on GSH and GPx after PostTranscriptional Gene Silencing in Mouse Skeletal Muscle Cell

WANG Xiaolong 1,2，XU Kai 1，QIN Ouju 1，YANG Chuanping 2*
   

  1. 1. Wildlife Medicine Dept., College of Wildlife Resource, Northeast Forestry University, Harbin 150040，China; 2. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-24 Published:2009-07-24
  • Contact: YANG Chuanping

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Abstract: The expression of Selenoprotein W (SelW) in C2C12 skeletal muscle cells was specifically decreased to examine its influence on the amount of glutathione (GSH) and the activity of glutathione peroxidase (GPx). SelW knockdown was performed by RNA interference (RNAi) in cultured muscle cells and verified by Realtime PCR and Western blotting. In addition, cell viability, GSH content and GPx activity were assayed. The results showed that the mRNA level and protein expression of SelW were decreased successfully by 719% and 688% relative to control values. WST assay showed that to compare with blank control, the value of positive group dropped 215%; In GSH and GPx assay, to compare with blank control the positive group increased 2976% and 4758% separately. In conclusion, SelW knockdown by RNAi caused significant cytotoxity in skeletal muscle cells and led to compensatory increases in GSH content and GPx activity. These findings are consistent with the suggestions from bioinformatics indicating an antioxidative role for SelW in skeletal muscle cells.