ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (5): 775-779.doi:

• 研究简报 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the rDNA-ITS of Parabronema skrjabini

ZHANG Xiao-dong1, YANG Xiao-ye1*, YANG Lian-ru1, LI Lin-chuan2,
ZUO Hai-tao3, NA Ren-hua3, ZHAO Zhi-guo1,WANG Jun-jie1   

  1. 1. College of Animal Science and Animal Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China;2. Inner Mongolia Center for Animal Disease Prevention and Control, Huhhot 010020, China;3. Veterinary Workstation of Alashan League, Alashan Left County 750306, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

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Abstract: The internal transcribed spacer （ITS1, 5.8S and ITS2）of ribosomal DNA nucleotide fragments of Parabronema skrjabini isolated from the camel of Inner Mongolia region were amplified by PCR using three pairs of conserved primers NC5, NC13, NC13r and NC2. The PCR fragments were purified and cloned into pGM-T vector. The inserts were successfully sequenced, and the results revealed that the first sample′s (P.sk1) ITS inserts were 837 bp in length and consisted of partial 18S, 28S and complete ITS1（298 bp）, 5.8S（157 bp） and ITS2 （281）rDNA sequences. The second sample’s (P.sk2) ITS1 inserts were 372 bp in length and consisted of partial 18S, 5.8S and complete ITS1（296 bp）. The third sample’s (P.sk3) ITS2 inserts were 484 bp in length and consisted of partial 5.8S, 28S and complete ITS2（284 bp）. ITS2 sequences shared 30.2%-60.1% homology with other nematodes. It was the first time that the complete sequence of ITS and 5.8S rDNA of P. skrjabini were reported. The results of this study laid a foundation for further studies.