Abstract: The polymorphism of the 5′regulatory region of Myostatin gene was detected using PCR-RFLP method in 45 Jinpi F2 pigs.The results showed that there were 3 genotypes (AA, TT and AT) and their frequencies were 0.244, 0.311 and 0.444, respectively. Growth, carcass traits and Longissimus dorsi muscles analyzed using immunohistochemical were recorded. The results showed that the DraI polymorphism of MSTN gene had significant effect(P<0.05) on the muscle fiber diameter, the percentage of MHC type I fiber, the backfat thickness (shoulder)，the average daily gain from birth to 4 months old and the eye muscle lightness.