ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (2): 161-165.doi:

• 遗传繁育 • Previous Articles     Next Articles

Tissue Distribution, SNP Detection and Its Association Analysis with Carcass and Meat Quality Traits of Chicken MyoT Gene

ZHU Zhi-ming1,2, CHEN Guo-hong3, QIANGBA Yang-zong4, LI Chang-chun1,
ZHU Meng-jin1, FAN Bin1, LIU Bang1*   

  1. 1.Lab of Molecular Biology and Animal Breeding,College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070，China；2. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences,Fuzhou 350013，China; 3. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009，China;4. College of Agriculture and Animal Husbandry, Tibet University, Linzhi 860000，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-02-24 Published:2009-02-24
  • Contact: LIU Bang

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Abstract: The semi-quantitative RT-PCR was performed to detect the expression pattern of chicken MyoT gene in 7 different tissues (heart, liver, spleen, lung, kidney, brain and muscle). The results showed that MyoT gene was expressed only in heart and muscle. Partial DNA sequence of chicken MyoT gene was cloned and a A/G transition was found in exon 10. Using PCR-Hin6Ⅰ-RFLP method, the A/G SNP locus was analyzed in 12 native chicken breeds (White Ear, Xianju, Dagu, Luyuan, Gushi, Game chickens, Silky Sooty, Beijing Fatty, Chahua, Xiaoshan, Langshan and Tibetan chicken) and 1 foreign breeds (Recessive White chicken). The association between different genotypes and carcass and meat quality traits was analyzed in Tibetan chicken, Recessive white, hybrid chicken (Tibetan♂×Recessive♀). The result of association analysis showed there were correlation between this SNP site and leg muscle weight (P＜0.05), and significant correlation between this SNP site and leg muscle rate and meat colour (leg muscle) (P＜0.01).