ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (12): 1788-1793.doi:

• 预防兽医 • Previous Articles     Next Articles

Development of an Indirect ELISA for Detecting Antibodies against Cysticercus cellulosae Based on Its Recombinant 18 ku Protein Expressed in E. coli

WU Guohua 1, ZHENG Yadong 1, JIA Wanzhong 1, ZHANG Shaohua 1, JING Zhizhong 1, LUO Xuenong 1,LIU Shiquan 2, CAI Xuepeng 1*
   

  1. 1. Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China; 2. College of Animal Science and Technology，Jiangxi Agricultural University，Nanchang 330045，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-12-24 Published:2009-12-24
  • Contact: CAI Xuepeng

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Abstract: A 18kDacoding gene of Taenia solium metacestodes was amplified by RTPCR and subcloned into pGEMT vector for sequencing. A recombinant plasmid pGEXCE18 was constructed and transformed into E. coli BL21 for in vitro expression. SDSPAGE and Westernblot were employed for analyzing the recombinant protein, which was used for development of an indirect ELISA for detection of anticysticercosis antibodies. The results showed that the interest protein was 35 kDa in size, accounting for 28% of total bacteria proteins, and could be recognized by positive sera against cysticercosis. Using the constructed indirect ELISA and a commercialized ELISA kit, paralleled analysis of 178 serum samples indicated that the concordant rate was 9883% and the ELISA showed good performance in specificity and sensitivity, supporting its application for cysticercosis diagnosis.