ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (8): 1033-1039.doi:

• 遗传繁育 • Previous Articles     Next Articles

Cloning, Sequencing and Distribution of Chick β-defensins Genes

MA De-ying1*，LIU Sheng-wang2, LI Yi-jing3，SHAN An-shan1   

  1. 1.Institute of Animal Nutrition, Department of Animal Science and Technology,Northeast Agricultural University, Harbin 150030,China;2.Division of Avian Infectious Disease, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001 ,China;3.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-08-24 Published:2008-08-24
  • Contact: MA De-ying

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Abstract: Three Gallinacin (Gal) genes: Gal-6, Gal-8 and Gal-9 were cloned from liver and tongue of chicks by RT-PCR, respectively. In addition, we have sequenced these genes. The full cDNA length of Gal-6 and Gal-9 consist of 201 bp encoding 67 amino acids, while Gal-8 consist of 204 bp encoding 68 amino acids. Differential gene expression of Gal-6, Gal-9 and Gal-8 has been demonstrated across a panel of tissues in the chick. It has been shown that Gal-6 mRNA was highly expressed in the skin, tongue, liver, heart, small intestine, breast muscle, kidney, bursa of Fabricius, spleen, and pancreas, and moderately expressed in the lung, testis, and borrow marrow. Gal-8 mRNA was highly expressed in the liver, tongue, small intestine and lung of chick. While, Gal-9 mRNA was highly expressed in the tongue and small intestine, and moderately expressed in the trachea, lung, liver, kidney, borrow marrow and thymus of chick.