Abstract: An experiment was conducted to breed low-excretion type Japanese quails for decreasing the environmental pollution source. Japanese quails from Normal ，Brown breeds and crossing group were used in this eight generations (6-20 week-old) selection experiment. Quails were allocated into selected and nonselected groups with 18% CP diets and control group with 24% CP diet, with feed intake as main select traits and laying rate and nitrogen excretion as subsidiary select traits. The results showed that adaptive capacity of selected and non-selected groups were both increasing with the increasing generation (P<0.05) and the genetic improving capacity of N strain was 0.26. Laying rate between generations had an increasing trend, the average cumulative laying rate of selected group was higher than that of non-selected group (P<0.05), and the genetic improvement of selected group was significant (P<0.01). There were no difference on feed intake between generations(P>0.05), while the feed intake of selected groups was higher than that of non-selected group but lower than that of control group (P<0.05) in the same generation. The nitrogen excretion and serum uric acid content of selected group was lower than that of non-selected and control group after improved two generations (P<0.05), and the nitrogen excretion was degreed by 15%-17%. To sum up, laying performance was improved and nitrogen excretion was reduced of Japanese quails in the N and B breeds after selective breeding, especially in BN group.