ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (12): 1743-1747.doi:

• 预防兽医 • Previous Articles     Next Articles

Construction and Identification of the Expression Recombinants for PEDV COE Gene in Lactococcus Lactis

DONG Li-na1, GAO Feng-shan2, XU Chong-bo2, HU Gui-xue1*，JIANG Hai-long1   

  1. 1.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2.College of Bio-engineering, Dalian University, Dalian 116622, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-12-24 Published:2008-12-24

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Abstract: The partial S glycoprotein gene of porcine epidemic diarrhea virus (PEDV) was amplified by RT-PCR and sequenced. The gene consisted of 531 bp and shared 99.4% nucleotide homology with PEDV CV777 strain (AF353511) published on GenBank. The COE gene (about 501 bp) in S glycoprotein gene was subcloned into the Lactococcus lactis vectors pNZ8149 and transformed into Lactococcus lactis NZ3900 by electroporation. The recombinant protein was detected by Western blot and IFA experiments after the bacteria were induced by 1 ng/mL nisin. The results indicated that the molecular weight of the expressed recombinant protein was about 20 kDa; The protein had the reactionogenicity with antibody against PEDV as expected；The protein was secreted and located on the surface of the bacteria.