Abstract: Foot-and-mouth disease virus（FMDV）has been showed at least to use four integrins, αvβ1, αvβ3, αvβ6 and αvβ8 as receptors to initiate infection. In this study, the integrin αv genes were cloned from the lung tissue of cattle and pig, both infected experimently with FMDV, healthy sheep and Bactrian camel by RT-PCR. The nucleotide and predicted amino acid sequences of the αv genes of sheep, cattle, pig, Bactrian camel, human, dog, horse, chimpanzee, rhesus monkey, chicken, and mouse were analyzed. The ORF of integrin αv cDNA of sheep, cattle, pig, Bactrian camel consists of 3 147 nt, 3 147 nt, 3 141 nt, 3 165 nt, encoding a polypeptide of 1 048 aa,1 048aa, 1 046 aa, 1 054 aa, respectively. The putative signal peptide, transmembrane domain and cytoplasmic domain consists of 30, 29, 32 residues, respectively. The ectodomain of integrin αv contains 957 aa, 957 aa, 955 aa, 963 aa in sheep, cattle, pig, Bactrian camel, respectively. The homology analysis showed that the signal peptides exhibit the lowest conservation, and the transmembrane domain and cytoplasmic domain exhibit the highest degree of conservation, and the degree of similarity was somewhat higher for ectodomain. Phylogenetic analysis revealed that integrin αvs of sheep, cattle, pig, Bactrian camel belonged to the same branch, suggesting that they had a close genetic relationship. It is possible that host and tissue tropism of FMDV may related to divergence in αv subunit among different species.