ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (11): 1517-1522.doi:

• 动物营养 • Previous Articles     Next Articles

Effect of Daidzein and Genistein on Proliferation and Antioxidation of Mammary Epithelial Cell of Dairy Cow in vitro

LIU Chun-long1,2 ，LI Zhong-qiu3，ZHANG Fan1，JIANG Wen-bo1 ，
XU Yan1，SHAN An-shan1*   

  1. 1. Animal Nutrition Institute,Northeast Agricultural University, Harbin 150030, China; 2. Heilongjiang Key Laboratory of Blacksoil Ecology,Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081,China;3. Animal Science Research Centre,Heilongjiang Academy of Agricultural Research,Harbin 150086,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-24 Published:2008-11-24

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Abstract: Effect of Daidzein and Genistein on the proliferation and antioxidation of mammary epithelial cell of dairy cow were determined in vitro. The experiments were assigned into 9 groups(blank,1,10,100,1 000 ng/mL Daidzein and Genistein).According to MMT,the proliferation experiment results showed that 10,100 ng/mL Genistein and 100,1 000 ng/mL Daidzein significantly improved dairy cow mammary epithelial cell proliferation(P＜005),but all weaker than 10 ng/mL E2 group (P＜0.05) after 72 h cultured. In the other experiment,the mammary epithelial cell during the logarithm growing period were collected after 24 h cultured to measured the TSOD,GSH-PX activity,MDA,NO content.Results showed that 100,1 000 ng/mL Daidzein and 100,1 000 ng/mL Genistein significantly increased the T-SOD activites and decreased MDA concentration (P＜0.05). 100,1 000 ng/mL Daidzein and 10,100, 1 000 ng/mL Genistein significantly increased NO concentration(P＜0.05).1 000 ng/mL Daidzein and 100,1 000 ng/mL Genistein significantly increased the GSH-PX activites (P＜0.05).It was proved that proper levels of Daidzein and Genistein can advance the proliferation and antioxidation of mammary epithelial cell of dairy cow.