Abstract: Seventytwo Landrace × Rongchang crossbred barrows, with initial body weight of 20.7 kg, were allocated to three treatments to study the effect of energy levels of dietary on mRNA expression for the content of intramuscular fat and their association with fatty acid synthase (FAS) and hormone-sensitive lipase(HSL) at finishing stage. The digestible energy levels of dietary were 11.75, 13.05 and 14.36 MJ/kg, respectively. The pigs were slaughtered at 100 kg body weight. The results showed that: ①There were no significant effects of energy density on average daily gain(ADG) and average daily feed intake(ADFI) (P>0.05), while feed conversion ratio was improved(P<0.05)and the duration of finishing was shortened (P < 0.05) with energy density increasing. The percentage of external fat, backfat thickness and intramuscular fat were increased (P < 0.05) with increasing of energy density; ②The dietary energy density of 14.36 MJ/kg improved the mRNA expression of FAS in backfat (P < 0.05) , decreased the mRNA expression of HSL (P < 0.05) and increased the ratio of mRNA of FAS to HSL (P < 0.05) in both backfat and intramuscle; ③There were significantly positive correlations (P < 0.05) between energy levels and intramuscular fat, FAS mRNA and the ratio of mRNA of FAS to HSL, between the ratio of mRNA of FAS to HSL and intramuscular fat . However, the extremely significantly negative correlations (P < 0.01)were observed between HSL mRNA and dietary energy，significantly negative correlations (P < 0.05) were observed between HSL mRNA and intramuscular fat. This study indicated that higher energy level of dietary could increase fat deposition. Moreover, effects of dietary energy level on fat deposition, especially on intramuscular fat deposition, maybe modulate both the mRNA of FAS and HSL, not anyone of them.