ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (5): 476-481.doi:

• 预防兽医 • Previous Articles     Next Articles

Development of TaqMan fluorescence quantitative RT-PCR assay for detection of transmissible gastroenteritis virus of swine

BAI Xing-hua1;FENG Li;CHEN Jian-fei;SHI Hong-yan;SUN Dong-bo;WU Bo-ping;GAO Xiu-chun   

  1. 1. Department of Swine Infectious Diseases,National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China;2.Northeast Agricultural University, Harbin 150030,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-25 Published:2007-05-25

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Abstract: The primers and probes were designed and synthesized according to the sequences of transmissible gastroenteritis virus and β-actin, and then reaction parameters were optimized to develop a TaqMan fluorescence quantitative RT-PCR assay. Meanwhile,37 field samples were detected and the results were compared with that of routine RT-PCR and antigen rapid test kit. It was showed that the fluorescence quantitative RT-PCR assay could detect 15.3copies/µL of plasmid DNA and its specificity and reproducibility were very good，while the sensitivity of the routine RT-PCR was 1.53×103copies/µL. The result of field test also showed that its sensitivity was higher than that of the routine RT-PCR and TGEV antigen rapid test kit (chromatographic immunoassay).