Abstract: Plasmid containing EGFP gene was transfected into the goat mammary gland epithelial cells by lipofection. Stable transfectants were selected by G418 and the positive cells were used as nucleus donors to create transgenic goat cloning embryos by nuclear transfer techniques. SOFaa culture medium supplied with 10 % normal goat serum (NGS) was the best combination for culturing the transgenic goat cloning embryos in vitro. Expression of the foreign gene in the embryos was detected by observation of green fluorescence. EGFP was expressed gradually after the 816 cell stages in most embryos, and the level of EGFP expression increased with development of the embryos. The result implied that EGFP gene could be used as reporter gene to check expression of foreign gene in the transgenic goat cloning embryos in their early stages.