Abstract: The BMPR-IB gene, which is related to prolific traits of animal, was cloned by RT-PCR from the ovary tissue of Yunling black goat. The results showed that the amplified fragment sequence of the BMPR-IB gene was 467bp in length and it lied between the 497th base and the 963th base of the coding region of BMPR-IB. The homology of the amplified fragment sequence with the same coding region of the BMPR-IB gene in the wild type goat, sheep, pig, human and rat was 99%, 99%, 92%, 92% and 87%, respectively. Compared to the wild type goat, there were two polymorphic sites at the 498th and the 575th base of the nucleus acid sequence of the amplified region of BMPR-IB gene in Yunling black goat, which was that the T base mutated to C base at the 498th site and coding the same amino acid (aspartic acid, Asp), the C base mutated to T base at the 575th site and the coding amino acid changed from praline to leucine, and which might lead to the different secondary structure of BMPR-IB protein. These results would be the foundation for further researching the association between BMPR-IB gene and prolific traits of Yunling black goat.