Abstract: To elongate the proliferation life-span of porcine arterial endothelial cell(PAEC) in vitro. The eukaryotic expression plasmid pCI-neo-hTERT was transfected into PAEC by lipofection. FactorⅧ related antigen and CD34 of PAEC were identified after transfection by immunohistochemical method; we also detected telomerase activity of PAEC after transfection by PCR-ELISA. The results showed that Factor Ⅷ related antigen and CD34 in PAEC is positive after transfection. The PAEC after transfection expressed the vascular endothelial cell-specific protein still; Telomerase activity in PAEC is higher by hTERT introduction, the cell proliferation life-span was elongated to 20 population doublings compared to control. Conclusion, Telomerase activity can be activated in PAEC, thus the cell proliferation life-span was elongated in vitro.