Abstract: CD58 plays an important role in mammal immune system. In this study, a pair of primers was designed according to human and sheep CD58 sequence and used to clone porcine CD58 gene by RT-PCR method. In addition, the protein structure of porcine CD58 were predicted through bioinformatics methods and compared with human and sheep CD58. The results showed that an 800 bp fragment of porcine CD58 cDNA with an ORF of 735bp was obtained. Then, this cDNA was ligated into pGEX-4T-1 and transformed to E.coli BL21 cells. The recombinant transformant was induced with IPTG and the expressed product could be recognized by anti-CD58 serum. The prediction of the protein structure indicated that the porcine, sheep and human CD58 have high similarity, especially in V region which was the molecular foundation of xeno-adhesion between lymphocyte and RBC. This research will help us to develop new adjuvant and/or immune-regulated drugs, and laid a solid foundation for further study of structure of CD58 molecule and the mechanism of CD2-CD58 complex triggering immune system.