Abstract: The cDNA and fourth intron sequences of porcine NPM1 gene were cloned, and verified by bioinformatics method. The cDNA fragment was 1 195 bp with an entire open reading frame （ORF）, encoding 294 amino acids. The intron fragment was 300 bp and splice junctions follow ‘GT/AG’ rule. The analysis of sequence revealed that the porcine NPM1 gene was highly homologous with that of other species such as human （95%）, mouse （91%） and rattus norvegicus （91%） in amino acid. The protein encoded by porcine NPM1 gene had a conserved domain ‘Nucleoplasmin＇. This gene was assigned to SSC16q21 region and closely linked to SW977 with the LOD of 9.16 by SCHP and IMpRH mapping, respectively.