ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (12): 1405-1409.doi:

• 研究简报 • Previous Articles     Next Articles

Analysis of Immune Responses Induced by Recombinant Secretion and Non-secretion Type Lactobacillus casei Expressing Porcine Parvovirus VP2 Protein via Oral Immunizations

1. College of Veterinary Medicine,Northeast Agricultural University,
Harbin 150030，China; 2. Northeast Forestry University, Harbin 150040，China   

  1. 1. College of Veterinary Medicine,Northeast Agricultural University, Harbin 150030，China; 2. Northeast Forestry University, Harbin 150040，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

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Abstract: The objective of this research was to compare the immune responses induced by mucosal immunizations with recombinant secretion and nonsecretion type Lactobacillus casei expressing porcine parvovirus (PPV) protective antigen VP2 protein. The mice of vaccine groups received recombinant L. casei strains constructed in this work harboring cell surface expression system pPG-VP2 or secretion expression system pPGS-VP2, respectively. The control mice were immunized with equivalent doses of PBS. All the mice were immunized via orogastricroute. The immune protocol was administered on three consecutive days at day 0, 1 and 2. A booster immunization was given at day 14, 15 and 16 and a second booster was given at day 28, 29 and 30. Specific anti-PPV-VP2 secret immunoglobulin A (sIgA) antibody in the feces and intestines mucus and anti-PPV-VP2 IgG antibody in the serum of immunized mice was detected by indirect enzyme linked immunosorbent assay (ELISA) after orogastric immunization. The results showed that the levels of mucosal and systemic immune responses induced by secretion type recombinant lactobacilli were higher than that induced by cell surface expression type recombinant lactobacilli.