ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (6): 592-596.doi:

• 基础兽医 • Previous Articles     Next Articles

Construction of Recombinant Adenovirus Expressing Porcine Growth Hormone Gene and Study on Its Promoting Growth Function in pigs

CAO Han-wei;ZHANG Chun-hong;HUANG Zhong;RUAN Liang-ji;LIN Jie   

  1. 1.Institute of Veterinary Medicine , Guangdong Academy of Agricultural Sciences Guangzhou 510640, China; 2.Zengcheng Municipal Service Center of Animal Husbandry, Zengcheng 511300;China; 3.Deptartment of Microbiology and Immunology, University of Melbourne, Australia
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-06-25 Published:2006-06-25

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Abstract: A recombinant replication-defective human adenovirus serotype 5 containing the porcine growth hormone gene (Ad-pGH) was constructed with a homologous recombination method. A high level expression of pGH was detected in HEK293 cells and PK-15 cells infected with Ad-pGH. Furthermore, Ad-pGH were injected intramuscularly at a dose of 1 mL(1010TCID50) each to 60 day-old crossbred pigs.Compared with the control animals at 4 weeks and 6 weeks after the injection, the pigs increased in weight gain by 37% and 19% respectively. The feed/gain ratio improved in Ad-pGH treated pigs. These results suggested that Ad-pGH is able to promote growth in pigs significantly. The in vivo expression of pGH can be maintained about four weeks.