ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (6): 580-586.doi:

• 预防兽医 • Previous Articles     Next Articles

cnThe Immunological Efficacies of Recombinant Fowlpox Virus Expressing the S1 Gene of LX4 Strain of Infectious Bronchitis Virus in Specific-pathogen-free (SPF) Chickens

TIAN Zhan-cheng;SUN Yong-ke;WANG Yun-feng;ZHI Hai-dong;LIU Sheng-wang; SUN Hui-ling;WANG Mei;TONG Guang-zhi
  

  1. 1. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001,China; 2.Inner Mogolia of Agricultural University, Huhhot 010018,China; 3.China Agricultural University, Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-06-25 Published:2006-06-25

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Abstract: Sixty-four 4-week-old SPF chickens were randomly divided into 4 groups, and were inoculated with the parental fowl poxvirus (S-FPV-017), rFPV-IBVS1, live IB vaccine (H120) and PBS (control) respectively. Chickens immunized with rFPV-IBVS1 developed antibodies against IBV post-immunization. Four weeks post-vaccination, all chickens were challenged with a lethal dose (1×103.0 EID50/0.2 mL) of virulent IBV LX4 strain. The results showed that 12 out of 16 chickens immunized with rFPV-IBVS1 were well protected, and 13 out of 16 chickens were protected in the group immunized with IB vaccine. After challenge, chickens of rFPV-IBVS1 and H120 vaccinated groups had a shorter time period of virus shedding from respiratory tract in comparison with control group, and the virus separation rates were significantly lower than control group. Slight pathological changes could be seen in liver, spleen, kidney, lung and tracheal of chickens in H120 and rFPV-IBVS1 groups rather than in control group.