ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (4): 396-400.doi:

• 研究简报 • Previous Articles     Next Articles

Analysis of TbTA1 Involved in Antrycide-resistance of Trypanosoma evansi

LIAO Dang-jin; SHEN Jie;DONG Wang-nian
  

  1. 1.Sichuan Academy of Animal and Science, Chengdu 610066, China;2.Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, Shanghai 200232, China; 3.Meishan Animal Bureau, Meishan 612100, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-04-25 Published:2006-04-25

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Abstract: For exploring mechanism of drug-resistance of Trypanosoma evansi to antrycide, the RT-PCR and PCR were performed to clone the TbTA1 from the sensitive strain（TeS） and the resistant strain（TeR） under the different conditions of annealing temperature. The TbTA1 was obtained from the cDNA and DNA of the sensitive strain, but not obtained from the antrycide-resistant strain of T. evansi. The results suggested that the TbTA1 in the drug-resistant strain had some changes which result in antrycide-resistance and the TbTA1 of T. evansi, therefore, is involved in antrycide-resistance of T. evansi. On the other hand, the comparison of sequence of the TbTA1 between T. evansi and T. brucei revealed the 10 different nucleotides, G88(T. e)-A(T. b), T144(T. e)-C(T. b), C224(T. e)-T(T. b), C471(T. e)-T(T. b), A472(T. e)-G(T. b), G549(T. e)-T(T. b), C1 008(T. e)-T(T. b), A1 033(T. e)-G(T. b), A1 293(T. e)-G(T. b) and T1 384(T. e)-C(T. b), resulting in Val30(T. e)-Ile(T. b), Ala75(T. e)-Val(T. b), Ila158(T. e)-Val(T. b), Thr345(T. e)-Ala(T. b) and Ser462(T. e)-Pro(T. b), these difference maybe due to different species.