ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (4): 368-373.doi:

• 基础兽医 • Previous Articles     Next Articles

Research on Expression of Hematopoietic and Neural Markers in Human Placenta

WANG Yue-si; MENG Fan-gang; JIANG Yu-zhu; LI Jian-yuan;FAN Guang-li
  

  1. 1. Institute of Biology Technical , Shandong Binzhou Medical College, Binzhou 256603,China; 2. College of Animal Science and Veterinary Medicine, Northwest A&F University, Yangling 712100, China ； 3. Shandong Research Center of Stem Cell Engineering , Yantai 264000,China；4 Department of Neurosurgery, QiLu Hospital of Shandong University, Jinan 250012，China；5 Graduate School of Immunology，Shandong University, Jinan 250012，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-04-25 Published:2006-04-25

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Abstract: The expression of neural and hematopoietic markers in placenta tissue and cultured human placenta derived adherent cells (hPDACs) and their correlation in embryonic development were investigated in this paper. The function of human placenta in hematopoiesis was demonstrated by immunocytological study in 7 and full-term months’placental slices. Positive reactions were observed in 7 month placenta using antibody against SCF, VEGF, BMP-4, FGF-1, CD34, CD133 and KDR. However, full-term months’placenta just reacted slightly with these antibodies. The staining for neural markers such as Nestin and MAP2 showed positive reactions in 7 month and negative in full-term placenta. GFAP was showed negative in all two groups placenta’s dying. The isolated adherent cells of placenta were positive for AKP, vitamin, SCF, VEGF, BMP-4, FGF-1, CD133, Nestin and MAP2 markers, while they were negative for CD34, GFAP and MBP. They showed neural differentiation potential under appropriate conditions. The data suggest that placenta can express hematopoietic related factors to support hematopoiesis, and placental-derived cells have neural differentiation potential.