ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (3): 295-298.doi:

• 临床兽医 • Previous Articles     Next Articles

Apoptosis of L1210 Cell Line Induced by Extract from Tephroseris Kirilowii Turcz Holub in vitro

CHEN Jin-jun;WANG Jian-hua
  

  1. 1．Department of Veterinary Medicine, Agricultural College, Guangdong Ocean University, Zhanjiang 524088,China；2．College of Animal Sciences, Northwest Sci-Tech University of Agriculture & Forestry, Yangling 712100,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-03-25 Published:2006-03-25

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Abstract: With a reference system of cyclophosphamide (CY), quercetin (QU) and monocrotaline (MO), the apoptotic cellular morphology of murine L1210 induced by the extract from Tephroseris kirilowii Turcz Holub with 60% ethanol solution (EX) was observed in vitro. The early apoptotic actions of EX to murine L1210 cells were studied by Annexin Ⅴ-FITC/PI of flow cytometry and the apoptotic mechanism of EX to murine L1210 was discussed. The results showed that EX at 1.0μg/mL made murine L1210 cells become apoptotic bodies in morphology and EX induced murine L1210 cells into early apoptosis with 86.8% Annexin Ⅴ-FITC+/PI-cells.