Abstract: The location and distribution of the primary sensory neurons on the hens’ uterus of uterine tube were determined by retrograde trace method．CBHRP solution was injected into the wall of the uterus of ten Issabrown hens. The results showed that the primary sensory neurons of Issabrownhens uterus of uterine tube were innervated by bilateral spinal ganglia from T₁ to LS₁₃，bilateral jugular and nodose ganglia．The numbers of labeled neurons in the left were more than those in the right，and that in the spinal were more than those in the jugular and nodose ganglia． A lot of labeled neurons in the spinal ganglia were accumulated on the segments T₅-LS₁ and LS8-LS11．The greatest concentration of labeled neurons were in T₇ and LS₁₁．It was revealed that hens’ uterus of uterine tube were innervated by bilateral spinal and vagus afferent nerves，in which mainly conducted by ipsilateral or left side spinal and vagus nerves，and the spinal was dominat，although the uterus of uterine tube of hens was the unilateral visceral organ．