Abstract: Eighty-three strains of Newcastle disease virus（NDV） were isolated from cases during epidemic in different parts of China in 2005. The main functional region of the F gene were amplified by RT-PCR and sequenced. All the sequences obtained from the NDV isolates were submitted to GenBank, the accession numbers were DQ439866-DQ439948.The sequence analysis showed that the length of the F gene segments amplified were 535 bp. For genotyping, phylogenetic tree based on the nucleotide 47-435 of the F gene were performed using sequences of 83 isolates and other NDV sequences obtained from GenBank. The results revealed that all the isolates belonged to 5 genotypes(Ⅶ,Ⅵ,Ⅲ,Ⅱ and Ⅰ). Most of the isolates (65 strains) were classified into genotype Ⅶ. There are 9 strains belonged to genotype Ⅱ, which were similar to the vaccine strain LaSota. Five strains were proved to be genetype Ⅰ, which were similar to V4. Three strains were genotype Ⅵ, and one isolate, NDV05-041, belonged to genotype Ⅲ. It indicated that the genotype Ⅶ NDV was the major pathogeny causing the epizootic of ND in 2005 in China.