Abstract: Liposome/DNA and chitosan/DNA complexes of infetious bronchitis virus which were prepared were immunized health chickens via intramuscular injection(i.m.)and eye drop, intranasal administration (i.n), with naked DNA vaccine as control. Fluorescence activated cel1 sorter(FACS) and indirect ELISA methods were used to detect the number of CD4+ and CD8+ T lymphocytes and antiIBV antibody IgG.All the chickens were challenged with 0.2 mL of 103EID50 IB SAIBK on day 35 after vaccination. Result: The number of CD4+ T lymphocyte of the experiment groups at 1-3 weeks post inoculation was higher than that of the control group (P<0.01 or P<0.05),and between the experiment groups, the number of CD4+ cell of liposome/DNA vaccine 10 days after inoculation was higher than that of chitosan/DNA vaccine via i.m, but only appeared significant difference at the 15th day, but it was opposite by i.n.administration. The number of CD8+ T lymphocyte of the experiment groups were higher than that of control group(P<0.01 or P<0.05)on day 15 after vaccination, however it was not different between the experiment groups. The level of specific IgG of all groups only appeared insignificant differece. The livability of chickens of liposome DNA group(i.m.)was 100%,other experiment groups had 4% or 8% mortality.The mortality of all experiment groups was lower that of control group(12%).From these results we can draw the conclusion that liposome and chitosan were good adjuvant or vector of DNA vaccine, and the immune responses of liposome/DNA vaccine was better than chitosan/DNA vaccine via i.m.