Abstract: To clarify regulation of INS, GLN and LP on the mRNA expression of HSL and LP in vitro neonatal calf adipocytes, different concentrations of INS (0, 5, 10, 20, 50 and 100 mmol/L), GLN (0, 25, 100, 200, 500 and 1 000pg/mL),and LP (0, 25, 5, 10, 50 and 100 ng/mL) were added respectively into media of primary monolayer culture calf adipocytes. After 12 h cultured, mRNA abundance of HSL and LP in the adipocytes was detected by FQPCR respectively. The results showed that INS plays a dosedependently inhibitory role in abundance of HSL mRNA and a stimulating role in LP mRNA in calf adipocytes in vitro. However, action of GLN was contrary to INS in abundance of HSL mRNA, and plays a no role in abundance of LP mRNA in calf adipocytes in vitro. With concentrations of LP, abundance of HSL mRNA in calf adipocytes in vitro first dosedependently increased, then decreased, but abundance of LP mRNA in calf adipocytes dosedependently decreased in vitro.