Abstract: In our study, we used 4 pairs of primers to clone Beijing duck myogenin cDAN from breast muscle of 42day, 14day old Beijing duck and leg skeletal muscle of 0day old bird, 22day, 15day old hatched duck embryo. However, target gene was not amplified from the the reversetranscribed products. Data analysis, revealed that myogenin was expressed during the period of forming the myoblast cells. Myogenin expression may be found in cultures of muscle satellite cells or denervated animal skeletal muscle. 260 bp partial nucleotide sequence of myogenin exton 1 was amplified from DNA of metioned tissuesIt coded for a predicted protein of 86 amino acids. The predicted amino acid sequence has conserved bHLH domain and an overall similarity with turkey and chicken. The results provided a major base for researching myogenin expression, cloning complete cds of myogenin and polymorphisms analysis of duck myogenin gene.