Abstract: The single strand gFSHβ-CTP-α gene was successfully constructed by the combination of the C-terminal peptide (CTP) of carboxyl-terminal region of human chorionic gonadotropin with the goat FSH α-subunit and β-subunit genes. The gene was subcloned into expression vector pVITRO and transfected into Chinese Hamster Ovary (CHO) cells after gene sequencing. The results show that FSH analogous gene expression vector was successfully constructed , the CHO cells expressed gFSHβ-CTP-α were acquired and the quantity of expression is 0.120 mIU/mL. These results would lay the molecular foundation for studying the relation between structure and function of CTP and the production of longacting FSH.