ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (9): 942-946.doi:

• 兽医 • Previous Articles     Next Articles

Effects of Earthworm Oligo-peptides on the Infection to Cell of PRV and HeLa Cell Apoptosis

LIU Yan-qin; SUN Zhen-jun;ZHANG Guo-zhong; LI Shi-jie   

  1. 1. College of Resources and Environmental Science, China AgriculturalUniversity, Beijing 100094, China；2. College of Animal Science, HebeiAgricultural Universtiy, Baoding 071000, China;3. Institute of Laboratory AnimalScience, China Agricultural University, Beijing 100094, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-09-25 Published:2005-09-25

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Abstract: In this paper, the CPE inhibition test was used to detect the antivirus effects, and the apoptosis effects on HeLa cell was studied by AO/EB staining method and DNA agarose electrophoresis. The results showed that the oligo-peptide could inhibit PRV infecting cells. At the same time, the effect had a dose and time dependent effect. The higher the concentration was, the smaller the CPE degree was, and the lowest CPE occurred when treated for 72 h. It verified that oligopeptide could lead to apoptosis of HeLa cell. The apoptotic effect was dose dependent. The apoptotic rates of HeLa cell in the 1.5mg/mL, 0.75 mg/mL group were 60.20% and 48.12% respectively.