ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (7): 649-653.doi:

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Study on mtDNA Cyt b Gene of Chinese Main Indigenous Sheep Breeds Using PCR-RFLP

GONG Yuan-fang;LI Xiang-long;LIU Zheng-zhu
  

  1. 1.Department of Animal Science, Hebei Normal University of Science & Technology, Changli 066600,China；2. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-07-25 Published:2005-07-25

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Abstract: Technique of polymerase chain reaction(PCR) and method of restriction fragment length polymorphism(RFLP) were performed to analyse Cytochrome b(Cyt b) gene of mtDNA in 9 indigenous sheep breeds and 3 imported sheep breeds.The results indicated that 22 restriction sites and 15 restriction morphs were detected with the digestion of 6 endonucleases in all individuals,and StuⅠ, EcoRⅠ and MboⅡ were polymorphic.Fifteen restriction morphs were sorted into 4 haplotypes.The haplotype I was the main haplotype.The averaged polymorphic degree(π value=0.014 2%) of mtDNA Cyt b gene showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very poor.